AP BIO pGLO transformation lab question?
If the genetically transformed cells have acquired the ability to live in the presence of the
antibiotic ampicillin, then what might be inferred about the other genes on the plasmid
that you used in your transformation procedure?
Answers & Comments
Verified answer
If they have acquired the resistance to the antibiotic from the gene on the plasmid, you can infer that most likely the other genes on the plasmid have been transformed into the cells as well, though it is best to independently confirm the presence of the genes if it is possible, using PCR or other expression markers.
That means the plasmid had the genes to give bacteria ampicillin resistance. Since plasmid was incorporated into the native Dna of the bacteria now the bacteria has ampicillin resistance which was introduced through the plasmid
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