Serial Dilution Problem?
You are asked to dilute a 80 mg/ml stock solution of sucrose. First, you add 7 mL of water (diluent) to tube 1 and add 7 ml of water to tube 2. Next, you transfer 0.5 mL of the sucrose stock solution into tube 1 and mix. Lastly, you transfer 1 mL from tube 1 into tube 2 and mix.
What is the concentration of sucrose in tube 2? Fill in the blank with the correct answer rounding to one decimal point.
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After adding 0.5 ml of sucrose sol. to 7 ml in tube 1, now the total sol. is 7.5 ml with 40 mg of sucrose.(because 1 ml contains 80 mg,so 05 ml has 40 mg.) After mixing within tube 1,now 1 ml of this sol has 5.33 mg of sucrose.(7.5 ml has 40 mg,so find for 1 ml).Therefore you are transferring 1 ml from tube 1 (in terms os sucrose it is 5.33 mgs) into tube 2,So the total vol. in tube 2 is 7+1=8 ml which contains 5.33 mgs. of sucrose.So find for 1 ml (to find the concentration) = 0.627mg/ml.