Serial dilution question?
Have to prepare for a lab but im not sure how to do the calculations:
You are provided with a 200ppm solution of nitrate. using 100ml volumetric flasks, prepare 100ml of the following by serial dilution:
20ppm nitrate
2ppm nitrate
0.2ppm nitrate
also prepare 10ml of 5ppm, 1ppm and 0.5ppm solutions from the 20ppm one.
Thanks
Answers & Comments
Verified answer
The serial dilution is easy. You decrease the concentration by a factor of 10 every time, so you dilute 1 in 10. For 100 mL volumetric flasks, that means 10 mL to 100 mL. So 10 mL 200 ppm to 100 mL will give you 20 mL, etc.
For the 10, 5, 1 and 0.5 ppm, work out the dilution factor. 20 → 10 means 1 in 2, so for 100 mL, you dilute 50 mL of 20 ppm to 100 mL to get 10 ppm. 20 → 5 is 1 in 4, so 25 mL to 100 mL. 1 ppm is 1 in 20, so 5 → 100. And 0.5 ppm is 1 in 40, so 2.5 → 100 mL
Hope that helps a little.
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