An easy genetics question?
You want to amplify a 2100 bp gene from genomic DNA using PCR. The sequence of the two ends of the coding strand is as follows:
5’- GAGGTACCATTTGAACATGA . . . [2060 bp] . . . AGTTATCCAGCTATATGACC -3’
Write the sequences of the two 20-base primers you would use to amplify the entire gene.
5’-______________________-3’
5’-______________________-3’
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Verified answer
Forward primer: 5’- GAGGTACCATTTGAACATGA'
5' AGTTATCCAGCTATATGACC 3'
Complementary: 3' TCAATAGGTCGATATACTGG 5'
Reverse primer: 5' GGTCATATAGCTGGATAACT 3'
There might be some mistakes in these base pairs, but you get the drift on how to establish primers for PCR.