Biology Pre-lab help?
I have a standard curve to answer this question...I just don't know where to start on it. I'm not sure what I'm missing...
1. You have two tubes (I and II) that initially contain the same amount of startch. However, you add one microgram of amylase enzyme to Tube II and then let both tubes sit for ten minutes. The resulting absorbance value for Tube I is 0.74 and for Tube II is 0.56. What is the starch concentration in both tubes? What is the rate at which the starch was digested (report in mg/ml per time).
I get I can find the starch concentration with the absorance values using the standard curve. But how do I figure out the rate of digestion without knowing what the values were before they sat for 10 minutes.
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As you don't add any amylase to tube 1 there will be no digestion. Since there will be no digestion the amount/concentration of starch will not change during 10 minutes. So if you look on your standard curve how much starch you have in tube one, you will have the amount tube 2 contained before you added amylase to it.
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