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Contractors don't usually negotiate. The best practice (from experience) is to get 3-5 estimates and go with one in the middle. Too low and you get what you paid for, too high is just screwing you - The middle guy is usually the best.

Be sure to get EVERYTHING (including estimated completion date) in writing or you will regret it.

No you do not counter offer. You get minimum of three bids from different contractors. You then negotiate with the one you can work with. The best you can say is the price is more than your budget and ask where some savings might. be. counter offering is futile since the only leeway a contractor has is on the cost of labor. If he agrees to your lower price, he will cut corners.

if you have not solicited bids from more than one contractor you are in a tough spot.

Negotiation is normal but you have little leverage without another contractor to use.

Bioluminescence is the production and emission of light by a living organism as the result of a chemical reaction during which chemical energy is converted to light energy. Its name is a hybrid word, originating from the Greek bios for "living" and the Latin lumen "light". Adenosine triphosphate (ATP) is involved in most instances. The chemical reaction can occur either inside or outside the cell. In bacteria, the expression of genes related to bioluminescence is controlled by an operon called the Lux operon. Bioluminescence has appeared independently several times (up to 30 or more) during evolution[1].

Bioluminescence occurs in marine vertebrates and invertebrates, as well as microorganisms and terrestrial animals. Symbiotic organisms carried within larger organisms are also known to bioluminesce.